ULTRASONIC HOMOGENIZER ideal for protein and enzyme extraction
 
UH SERIES

Ultrasonic Homogenizer, High efficiency and low cost laboratory homogenizer. Its powerful and reliable ultrasonic device for the sonication of larger samples in the lab. Typical application include: Homogenization, deagglomeration, lysis and cell disintegration, protein extraction and the emulsification of liquids.

* Novel design, Complete-function, Reliable ability
* Large LCD digital display, Fully microprocessor controlled and completely programmable
* Auto-tuning for convenience of use and optimal processing efficiency
* Temperature indicator and controller
* Power-emitted display for accuracy and repeatability, Variable power output, 0-950 watts
* Automatic warning system
* Integrated Sound Abating Chamber to reduce cavitational sound emitted during processing

UH-950
Technical Data
Power rate 950watt
Output Frequency 20-25 kHz
Process Timer 0-99min or Continues
Standard Process Sample Vol.
(Ø6mm Titanium Probe)
10mL - 100mL
Titanium Probe selection refer below**
Dimension 534X295X435mm
Weight 3200g
UH-1800
Technical Data
Power rate 1200watt
Output Frequency 19.5-20.5 KHz
Process Timer 0-99min or Continues
Standard Process Sample Vol.
(Ø20mm Titanium Probe)
500mL - 1000mL
Titanium Probe selection refer below**
Dimension 534X295X435mm
Weight 3200g

  Titanium Probe
Probe Power Frequency Capacity
Ø2mm 20-250W 20-25KHz 0.5 - 5mL
Ø3mm 30-400W 20-25KHz 0.5 - 5mL
Ø6mm 60-650W 20-25KHz 0.5 - 5mL
Ø10mm 100-950W 20-25KHz 0.5 - 5mL
Ø15mm 200-950W 19.5-20.5KHz 0.5 - 5mL
Ø20mm 400-1200W 19.5-20.5KHz 0.5 - 5mL
Ø25mm 800-1800W 19.5-20.5KHz 0.5 - 5mL

  FS Series
Model Frequency Power Probe Size Capacity (Volume)
FS-150 20 kHz 80W Ø 3 / 6mm 150uL ~ 50mL
FS-250 150W Ø 6 / 8mm 1mL ~ 100mL
FS-300 300W Ø 8 / 10mm 2mL ~ 200mL
FS-450 450W Ø 10 / 13mm 5mL ~ 300mL
FS-600 650W Ø 13 / 20mm 10mL ~ 600mL
FS-1200 1200W Ø 13 / 30mm 50mL ~ 2000mL

   APPLICATION
 

Listed below are sample applications and examples of use for the Ultrasonic Homogenizers. The list is not exhaustive and new applications are being found daily.

 

  Waste water samples
Aim: Homogenizing for determination of harmful substances, e.g. mineral oil, grease AOX in industrial and butcher’s waste water
Quantity: 250 ml
Approx. time: 5 - 10 min

Aluminium oxide suspensions
Aim: Dispersing
Quantity: 100 ml
Approx. time: ca. 4 min

Soil samples
Aim: Extraction for determination of pH value, Mg, K, P, N – contents for recommendation of fertilizer / determination of radio nucleides to control radioactivity in the environment (milk research)
Quantity: 50-100 ml / 100-150 ml
Approx. time: a few seconds

Meat and sausage samples
Aim: Homogenizing for determination of nitrates
Quantity: 100 ml
Approx. time: 3 min

Carbon black dispersions
Aim: Homogenizing
Quantity: 50 ml
Approx time: ca. 5 min 
Insect cells
Aim: Disruption for protein lay off
Quantity: 20 - 50 ml
Approx. time: app. 25 sec, pulsed

Liver tissue
Aim: Homogenizing for moleculargenetic tests
Quantity: 1,5 ml
Approx. time: 1½ min

Lymphocytes
Aim: Disruption
Quantity: 50 ìl - 2 ml
Approx. time: 1 - 5 min

Nano emulsions
Aim: Drop sizes within nm range
Quantity: 2 ml
Approx. time: ca. 5 min

Nano particles
Aim: Dispersing
Quantity: 1,5 ml
Approx time: 4 min

   SELECTION GUIDE
 
Ultrasonic Processing

Typical applications include sample preparation, cell lysing, disaggregation, homogenization, particle size reduction, soil testing, acceleration of chemical reactions, defoaming, and atomization.

The ultrasonic power supply (generator) converts 50/60 Hz voltage to high frequency electrical energy. This energy is transmitted to the piezoelectric transducer within the converter or tank where it is changed to mechanical vibrations.

These vibrations from the converter are intensified by the probe, creating pressure waves in the liquid. This action forms millions of microscopic bubbles (cavities) that expand during the negative pressure excursion and implode violently during the positive excursion. This phenomenon, called cavitation, produces the powerful shearing action at the probe tip and causes the molecules in the liquid to become intensely agitated.


Variables to Consider

The following variables must be considered to achieve efficient cell disruption with probe-style ultrasonic processors:

Probes: All probes, including those with replaceable tips, are tuned to resonate at 20 kHz, ±50 Hz. Low-surface tension liquids can penetrate the interface between the probe and the tip and carry Particulates into the threaded section, isolating the tip from the probe. If the tip is isolated or removed, the probe will not resonate at 20 kHz and the power supply will fail. To prevent this from happening, when processing low-surface tension liquids, such as solvents, always use a solid probe.

In ultrasonic processing, the higher the wattage and the larger the probe diameter, the greater the volume you can process. Also, the larger the tip diameter, the larger the volume you can process, but at reduced intensity.

Tip Amplitude and Intensity: Adding a tip to the end of the probe amplifies the longitudinal vibrations of the converter. Greater amplification, or "gain", creates more intense cavitation and greater disruption. Typical maximum tip amplitudes are 30 to 250 µm and the resulting output intensities are in the range of 200 to 2000 W/cm2 (compared to about 1 W/cm2 for ultrasonic cleaners).

Temperature: Cavitation is most effective at low temperatures. The intense agitation of ultrasonic processing can, over time, result in elevated temperatures that might damage sensitive biological samples. The temperature of your sample suspension should be as low as possible; ideally, the sample should be kept just above its freezing point. Use a cooling cell to prevent unwanted sample warming.

Power: Power is the energy required to drive the radiating surface of a given probe at a particular amplitude and frequency. Larger probes require higher power. While it is the intensity of cavitation that breaks the cells and emulsifies liquids (not the total power applied to the system), higher power is required to process larger liquid volumes and to process against higher liquid viscosities and pressures.

 
   
   
   

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